Background. For development of individualized treatment on a routine basis, transfer of patientsââ?¬â?¢ tumor tissue in a xenograftmodel\n(i.e., generation of patient-derived xenografts (PDX)) is desirable for molecular, biochemical, or functional analyses. Drawbacks\nare dissatisfactory tumor take rates, the necessity of fast tumor tissue processing, and extensive logistics demanding teamwork\nof surgeons, pathologists, and laboratory researchers. Methods. The take rates of ten colorectal cancer (CRC) tissue samples in\nimmunodeficient micewere compared after direct cryopreservation and after a 24 h cooling period at 4âË?Ë?C prior to cryopreservation.\nAdditionally, the effect of simultaneous Matrigel application on the take rates was investigated. Beside take rates, tumor growth\ncharacteristics and cell culture success were analyzed. Results. Tumor takes of CRC tissue samples were significantly improved after\nMatrigel application (8 versus 15 takes,
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